Venom composition of the Iberian adder (Vipera seoanei) from northern Portugal reveals limited sex and size variation

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  Venom composition of the Iberian adder (Vipera seoanei) from northern Portugal reveals limited sex and size variation Abstract Snake venom is a complex and dynamic ecological trait, with high potential for intraspecific variation. In this study, the venom composition of the Iberian adder ( Vipera seoanei ) from the Peneda Mountains of northern Portugal was investigated to explore whether venom varies with body size and sex. Venom proteomes from six individuals representing juvenile, subadult, and adult males and females were characterised using LC–MS/MS, while individual variation was assessed from SDS–PAGE profiles of 18 snakes. LC–MS/MS identified 144 proteins, dominated by phospholipases A 2 (PLA 2 ), snake venom serine proteases (svSP), and C-type lectin-related proteins (CTL). Descriptive comparisons revealed contrasting individual profiles, particularly between the juvenile male, characterised by high svSP and svMP abundance, and the juvenile female, characterised by high ...

In vivo biodistribution of Loxosceles intermedia venom in animal models with different pathological outcomes

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In vivo biodistribution of Loxosceles intermedia venom in animal models with different pathological outcomes

Abstract

Spiders of the Loxosceles spp. genus have a wide geographic distribution and are among the main causes of arachnidism in Brazil. Despite advances in venom characterization, fundamental aspects such as its in vivo kinetics and biodistribution remain little understood. This study aimed to investigate the biodistribution of Loxosceles intermedia spider venom through radiolabeling with technetium-99 metastable (99ᵐTc). Radiolabeling efficiency was evaluated using paper chromatography and C18 column, and effectiveness was assessed by SDS-PAGE. Biological activity after radiolabeling was verified through an in vitro sphingomyelinase activity assay and by evaluating dermonecrotic and edematogenic effects in rabbits. The results showed that most venom proteins were efficiently radiolabeled and that the venom remained biologically active after radiolabeling, allowing its distribution to be tracked in mice and rabbits. In both models, the venom concentrated at the injection site and was predominantly biodistributed to the kidneys and bladder. These findings are relevant given the distinct pathophysiological profiles of the animal models used. This is the first study to evaluate, in real time and in live animals, the biodistribution of L. intermedia venom through radiolabeling, and it may provide valuable data for understanding its mechanisms of action and improved the diagnostic of loxoscelism.
Da Silva Moreira, R., Pires, L. O., Cardoso Cunha, L., Fiorini de Carvalho, I. A., Chávez-Olórtegui, C., Minozzo, J. C., Rocha, M. N., Cardoso, V. N., Antunes Fernandes, S. O., & Felicori, L. F. (2025). In vivo biodistribution of Loxosceles intermedia venom in animal models with different pathological outcomes. Toxicon, 108619. https://doi.org/10.1016/j.toxicon.2025.108619