The faunistic diversity of the spiders (Arachnida, Araneae) of the Waterberg District in the Limpopo Province of South Africa

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  The faunistic diversity of the spiders ( Arachnida , Araneae ) of the Waterberg District in the Limpopo Province of South Africa Abstract The South African National Survey of Arachnida ( SANSA ) has conducted surveys in the Waterberg District of the Limpopo Province, South Africa, over more than 40 years. The first annotated checklist for the Waterberg District is provided here, along with the global distributions, endemicity and conservation assessments for each species, as per the International Union for Conservation of Nature ( IUCN ) criteria. A total of 54 families, 292 genera and 600 species were recorded. The study highlights species of special conservation concern, as well as the nine species endemic to the Waterberg District. Salticidae (95 species), followed by Thomisidae (80 species), Araneidae (53 species) and Gnaphosidae (48 species), are the most species-rich families. In contrast, single species represent 14 families. Most species (546; 91.2%) are widely distr...

Targeting Key Enzymatic Snake Venom Proteins Using Repurposed Small Molecule Inhibitors: Emerging Adjuncts to Antivenom Therapy

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Targeting Key Enzymatic Snake Venom Proteins Using Repurposed Small Molecule Inhibitors: Emerging Adjuncts to Antivenom Therapy

Abstract

Snakebite envenoming is a neglected tropical disease causing approximately 81,410–137,880 deaths globally each year and four-fold more disabilities than mortality rate. Despite the availability of antivenoms for treatment, several in vitro and in vivo preclinical studies have shown that their efficacy is limited by many factors including regional venom variation and poor neutralization of major venom toxins. To address these limitations, the potential of alternatives including aptamers, recombinant monoclonal antibodies, camelid antibodies, small molecule inhibitors, and natural product-based inhibitors targeting key venom proteins have been explored. Among these, small molecule inhibitors targeting key enzymatic snake venom proteins are emerging as adjuvants to antivenom treatment. Most of these small molecules are repurposed drugs with established safety, oral bioavailability, and lower cost compared to antivenoms. In this regard, this review tries to compile available information regarding the use of small molecule inhibitors to counteract envenomation with special emphasis on three major enzymatic snake venom protein families: phospholipase A2, snake venom metalloproteinases, and snake venom serine proteases. In vitro studies have shown that these small molecule inhibitors used either alone or in combination with antivenom can potentially reduce the adverse effects of venom-induced coagulopathy, neurotoxicity, tissue damage, and inflammation. However, critical gaps remain including limited human clinical trial data, uncertain efficacy across diverse venoms, and undefined dosing strategies. Overall, small molecules represent a mechanistically targeted and clinically promising adjunct to antivenom therapy, warranting further validation through randomized trials, pharmacokinetic studies, and development of field-applicable treatment protocols.

Reghu, N., Kiruthika, S., Santhosh, A. K., Narayanan, A. L., Geetha, K. G., Sidheek, N., Danthuluri, S. S., Nair, B. G., & Vanuopadath, M. (2026). Targeting Key Enzymatic Snake Venom Proteins Using Repurposed Small Molecule Inhibitors: Emerging Adjuncts to Antivenom Therapy. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms27136000