Venomics of the six-eyed sand spider, Sicarius rugosus (Araneae: Sicariidae), from the neotropical dry forest of Costa Rica

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  Venomics of the six-eyed sand spider, Sicarius rugosus (Araneae: Sicariidae), from the neotropical dry forest of Costa Rica Abstract Background Sicarius rugosus , the only member of the genus that inhabits Central America, is phylogenetically related to South American  Sicarius  spiders. These originated from a common ancestor with sister African species. Like  Loxosceles ,  Sicarius  exhibits venom phospholipase D activity due to a group of toxins known collectively as SicTox. Methods A gel-assisted, bottom-up, proteomic analysis was performed to characterize the venom composition of  S. rugosus . Hyaluronidase activity was determined using zymography. Results We identified several SicTox sequences, all classified as β-clade paralogs and sharing unique peptides with proteins from  S. patagonicus ,  S. peruensis,  and other species. Enzymes such as metalloproteinases, including putative astacins, carboxypeptidases, and angiotensin-conv...

Comprehensive molecular characterization and comparison of venom proteins and transcripts in three Gloydius species from South Korea

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Comprehensive molecular characterization and comparison of venom proteins and transcripts in three Gloydius species from South Korea

Abstract

In Korea, Gloydius brevicaudus, G. ussuriensis, and G. intermedius are the primary venomous species causing human envenomation. Although these species belong to the same genus, their envenomations induce distinct clinical symptoms, and the inconsistent efficacy of antivenoms underscores the need for species-specific therapeutic strategies. However, comprehensive molecular and toxicological profiling of Korean Gloydius species has remained unexplored. In this study, we quantitatively distinguished the venoms of three Gloydius species using two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. The results revealed that each species has evolved distinct venom protein profiles. To further investigate their expression regulation, we identified transcripts coding the major venomous proteins, which revealed species-specific regulatory differences at the transcriptional level. To verify the functionality of the identified transcripts, recombinant expression of two SVMPs was successfully expressed and their proteolytic activities were tested. This study establishes a systematic framework for understanding the molecular diversity of Korean Gloydius venoms and identifies species-specific toxin candidates that could serve as potential leads for the development of tailored antivenoms, diagnostic tools, and therapeutic vaccines.

Park, H.S., Moon, J.M., Chun, B.J. et al. Comprehensive molecular characterization and comparison of venom proteins and transcripts in three Gloydius species from South Korea. Sci Rep (2026). https://doi.org/10.1038/s41598-026-40454-1