On a new genus of dwarf tarantulas (Araneae: Mygalomorphae: Theraphosidae) endemic from Peru: evidence from morphology and molecular phylogeny, with description of three new species

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  On a new genus of dwarf tarantulas ( Araneae : Mygalomorphae : Theraphosidae ) endemic from Peru: evidence from morphology and molecular phylogeny, with description of three new species Abstract Recent field campaigns conducted in Peru along with the examination of museum specimens allowed us to identify small tarantulas that do not fit with any known Theraphosidae genera. Morphology and additional molecular evidence from the mitochondrial gene COI led us to propose Kiskalla gen. nov . from southern Peru, at Puno region. Three new species of Kiskalla gen. nov . ( K. ignacioi sp. nov ., K. yeisoni sp. nov . and K. zukuapasanka sp. nov .) are herein described, diagnosed and illustrated. Kiskalla gen. nov . differs from the known Theraphosinae genera in the presence of lateral stripes on the abdomen and a small dorsal arrowhead-shaped patch of type III urticating setae, presence of a large number of spines on all legs, short and stout setae on the dorsal metatarsi encirc...

Comprehensive molecular characterization and comparison of venom proteins and transcripts in three Gloydius species from South Korea

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Comprehensive molecular characterization and comparison of venom proteins and transcripts in three Gloydius species from South Korea

Abstract

In Korea, Gloydius brevicaudus, G. ussuriensis, and G. intermedius are the primary venomous species causing human envenomation. Although these species belong to the same genus, their envenomations induce distinct clinical symptoms, and the inconsistent efficacy of antivenoms underscores the need for species-specific therapeutic strategies. However, comprehensive molecular and toxicological profiling of Korean Gloydius species has remained unexplored. In this study, we quantitatively distinguished the venoms of three Gloydius species using two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. The results revealed that each species has evolved distinct venom protein profiles. To further investigate their expression regulation, we identified transcripts coding the major venomous proteins, which revealed species-specific regulatory differences at the transcriptional level. To verify the functionality of the identified transcripts, recombinant expression of two SVMPs was successfully expressed and their proteolytic activities were tested. This study establishes a systematic framework for understanding the molecular diversity of Korean Gloydius venoms and identifies species-specific toxin candidates that could serve as potential leads for the development of tailored antivenoms, diagnostic tools, and therapeutic vaccines.

Park, H.S., Moon, J.M., Chun, B.J. et al. Comprehensive molecular characterization and comparison of venom proteins and transcripts in three Gloydius species from South Korea. Sci Rep (2026). https://doi.org/10.1038/s41598-026-40454-1