Hidden in the woods: A new species of Hexura (Araneae, Mygalomorphae, Antrodiaetidae) from a highly restricted range in Idaho

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  Hidden in the woods: A new species of Hexura (Araneae, Mygalomorphae, Antrodiaetidae) from a highly restricted range in Idaho Abstract A new species of Hexura Simon, 1885 is described from a very restricted geographic range in the Rocky Mountains of Idaho, 500 km east of the previously known distribution of the genus in the Coastal Ranges and western Cascade Mountains of Oregon and Washington. This work provides the first taxonomic information for this group in almost 50 years, including a phylogenomic framework for the genus within the mygalomorph superfamily Atypoidea , based on Ultraconserved elements ( UCEs ). Hexura vandal sp. nov . can be distinguished from the other two described species by a combination of morphological, molecular, and geographic characteristics. Lastly, we discuss the need for conservation actions to secure the survival of the species. Calatayud-Mascarell A, Briggs EJ, Hamilton CA (2026) Hidden in the woods: A new species of Hexura (Araneae, Mygalomo...

Pioneering Comparative Proteomic and Enzymatic Profiling of Amazonian Scorpion Venoms Enables the Isolation of Their First α-Ktx, Metalloprotease, and Phospholipase A2

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Pioneering Comparative Proteomic and Enzymatic Profiling of Amazonian Scorpion Venoms Enables the Isolation of Their First α-Ktx, Metalloprotease, and Phospholipase A2

Abstract

Scorpionism is a growing public health concern in Brazil, with the Amazon region presenting the highest mortality rates but remaining understudied, especially regarding local scorpion venoms composition. This study presents the first comprehensive biochemical characterization of venoms from three Amazonian species—Tityus metuendus (TmetuV), Tityus silvestris (TsilvV), and Brotheas amazonicus (BamazV)—using an integrated approach combining Multi-Enzymatic Limited Digestion (MELD)-based bottom-up proteomics, high-resolution LC-MS/MS, chromatography, zymography, and enzymatic assays. Tityus serrulatus venom was included as a reference. Significant biochemical differences were observed: TsilvV was rich in 20–30 kDa proteins and showed strong metalloprotease activity; BamazV exhibited high molecular weight proteins and potent phospholipase A2 (PLA2) activity but lacked proteolytic and fibrinogenolytic activities; TmetuV showed the highest hyaluronidase activity and abundance of α-KTx neurotoxins. Zymography revealed a conserved ~45 kDa hyaluronidase in all species. Three novel components were partially characterized: BamazPLA2 (Group III PLA2), Tmetu1 (37-residue α-KTx), and TsilvMP_A (a metalloprotease homologous to antarease). This is the first application of MELD-based proteomics to Amazonian scorpion venoms, revealing molecular diversity and functional divergence within Tityus and Brotheas, emphasizing the need for region-specific antivenoms. These findings provide a foundation for future pharmacological studies and the discovery of bioactive peptides with therapeutic potential.

Bordon KCF, Santos GC, Martins JG, Wiezel GA, Amorim FG, Crasset T, Redureau D, Quinton L, Procópio REL, Arantes EC. Pioneering Comparative Proteomic and Enzymatic Profiling of Amazonian Scorpion Venoms Enables the Isolation of Their First α-Ktx, Metalloprotease, and Phospholipase A2. Toxins. 2025; 17(8):411. https://doi.org/10.3390/toxins17080411