Hidden diversity in plain sight: four new species of Nemesia (Araneae: Mygalomorphae) from the Córdoba Province of Spain

  Hidden diversity in plain sight: four new species of Nemesia (Araneae: Mygalomorphae) from the Córdoba Province of Spain Abstract A taxonomic revision of the species of Nemesia Audouin, 1826, distributed in the vicinity of Córdoba City, southern Spain, is presented. Four new species are described: Nemesia morana sp. nov. (female), N. kodama sp. nov. (male and female), N. rosae sp. nov. (male and female), and N. tamajoni sp. nov. (male and female). These species are distinguished by distinct morphological characters, including the shape of the spermathecae, palpal bulbs, and spinnerets, as well as by burrow architecture. Fieldwork was conducted across two of the three major habitat types in the region—the humid, forested mountains of Sierra Morena and the croplands and riparian forests of the Guadalquivir Valley—while the third, the “campiña”, which is mainly composed of agricultural landscapes, remains poorly explored. Sampling methods included pitfall trapping and the direct e...

Venom extraction method influences venom composition and potency in the giant house spider Eratigena atrica (C. L. Koch, 1843)

 


Venom extraction method influences venom composition and potency in the giant house spider Eratigena atrica (C. L. Koch, 1843)

Abstract

Extraction is the first step when investigating venom composition and function. In small invertebrates, widely used extraction methods include electrostimulation and venom gland extraction, however, the influence of these methods on composition and toxicology is poorly understood. Using the Giant House Spider Eratigena atrica as a model, we show that electrostimulation and venom gland removal extraction methods produce different protein profiles as assessed by Coomassie-stained SDS-PAGE and significantly different potencies in the cricket Acheta domesticus.
Lyons, K., Dugon, M. M., Boyd, A., & Healy, K. (2023). Venom extraction method influences venom composition and potency in the giant house spider Eratigena atrica (C. L. Koch, 1843). Toxicon, 234, 107303. https://doi.org/10.1016/j.toxicon.2023.107303