New spider species named after curator | Danni Sherwood | Manchester Museum

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New spider species named after curator | Danni Sherwood | Manchester Museum Two newly described spider species have been named in honour of members of our Entomology team, recognising their support for spider research at the Museum. Colombiarachne dianae, a tarantula from the Chocó region of Colombia, is named after our Curator of Entomology, Diana Arzuza Buelvas. Titanidiops logunovi, a trapdoor spider from Galegu, Sudan, is named after Dmitri Logunov. The names were chosen by one of our favourite frequent visitors, the amazing spider researcher Danni Sherwood. As Danni put it: "It’s a small token of my appreciation for all the help you give me in Manchester!" It's a wonderful reminder that behind every scientific discovery is a community of people helping to make research possible. Congratulations to Diana and Dmitri on this very special honour! 

The protamines of the noble false widow spider Steatoda nobilis provide an example of liquid-liquid phase separation chromatin transitions during spermiogenesis

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The protamines of the noble false widow spider Steatoda nobilis provide an example of liquid-liquid phase separation chromatin transitions during spermiogenesis

    Abstract

While there is extensive information about sperm nuclear basic proteins (SNBP) in vertebrates, there is very little information about Arthropoda by comparison. This paper aims to contribute to filling this gap by analyzing these proteins in the sperm of the noble false widow spider Steatoda nobilis (Order Araneae, Family Theridiidae). To this end, we have developed a protein extraction method that allows the extraction of cysteine-containing protamines suitable for the preparation and analysis of SNBPs from samples where the amount of starting tissue material is limited. We carried out top-down mass spectrometry sequencing and molecular phylogenetic analyses to characterize the protamines of S. nobilis and other spiders. We also used electron microscopy to analyze the chromatin organization of the sperm, and we found it to exhibit liquid-liquid phase spinodal decomposition during the late stages of spermiogenesis. These studies further our knowledge of the distribution of SNBPs within the animal kingdom and provide additional support for a proposed evolutionary origin of many protamines from a histone H1 (H5) replication-independent precursor.


The protamines of the noble false widow spider Steatoda nobilis provide an example of liquid-liquid phase separation chromatin transitions during spermiogenesis

Melissa R Leyden, Peter Michalik, Luciana Baruffaldi, Susheen Mahmood, Ladan Kalani, Donald F Hunt, Jose Maria Eirin-Lopez, Maydianne C.B. Andrade, Jeffrey Shabanowitz, Juan Ausió

bioRxiv 2024.06.04.597381; doi: https://doi.org/10.1101/2024.06.04.597381