Role of snake venom proteases and tissue factor in venom induced hemostatic disorders and local hemorrhage in mice

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  Role of snake venom proteases and tissue factor in venom induced hemostatic disorders and local hemorrhage in mice Abstract Bothrops jararaca venom (BjV) induces hemostatic and inflammatory manifestations. Tissue factor (TF) is essential for hemostasis, but aberrant TF expression can cause coagulopathy. We determined the effect of inhibition of BjV serine proteases and metalloproteinases, as well as host TF, on the systemic coagulopathy and local hemorrhage in envenomed mice. Venom serine proteases induced hypofibrinogenemia whereas venom metalloproteinases induced hemorrhage. TF did not contribute to venom-induced coagulopathy, but played a protective role in venom-induced bleeding in mice. Sachetto, A. T., Mackman, N., & Santoro, M. L. (2026). Role of snake venom proteases and tissue factor in venom induced hemostatic disorders and local hemorrhage in mice. Toxicon , 109299. https://doi.org/10.1016/j.toxicon.2026.109299

Comparison of two populations of the scorpion Centruroides mascota: venom composition and neutralization by a human recombinant antibody

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Centruroides mascota scorpion was described in 2011 by Ponce Saavedra's group as a species of medical importance in the state of Jalisco. In this study, we characterized two populations of scorpions collected in the states of Jalisco and Nayarit. Through a morphometric analysis, the determination of the LD50 of the respective venoms, electrophoresis, separation of their components by HPLC, mass spectrometry, and neutralization tests, it has been possible to establish that they correspond to the same species, distributed in different habitats. The scFv 10FG2 antibody fragment, which shows broad cross-neutralization of various scorpion toxins, was evaluated for the first time in the recognition of toxins from this venom and in its neutralization. The recombinant antibody fragment 10FG2 neutralizes 2 LD50 of venom of Centruroides mascota from both localities.

Gómez-Ramírez, I. V., Gonzalez-Santillán, E., Romero-Moreno, J. A., Fernández-Taboada, G., Güereca, L., Zamudio, F., Possani, L. D., Riaño-Umbarila, L., & Becerril, B. (2026). Comparison of two populations of the scorpion Centruroides mascota: Venom composition and neutralization by a human recombinant antibody. Toxicon, 109095. https://doi.org/10.1016/j.toxicon.2026.109095