Molecular Recognition and Immunopharmacological Implications of Serratiopeptidase Targeting Snake Venom Bradykinin-Potentiating Peptides

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  Molecular Recognition and Immunopharmacological Implications of Serratiopeptidase Targeting Snake Venom Bradykinin-Potentiating Peptides Abstract Purpose Bradykinin-potentiating peptides (BPPs), found in snake venom, are known to modulate cardiovascular functions, but their susceptibility to enzymatic degradation remains poorly understood. Methods Models were generated using AlphaFold-Colab for five small BPPs (< 9 amino acids) that did not have experimentally solved structures, and those BPPs which had reliable predictions of structure (pLDDT > 80) were used for docking studies. Protein-peptide docking experiments were done using HADDOCK for studying the interaction between wild-type (WT) and mutant serratiopeptidase with BPPs. After selecting the best complex, simulation of the complex was done using molecular dynamics (MD) simulations for 100 ns and binding energy determination using MM/PBSA analysis. For checking the mechanical stability of the protein-peptide complexes...

Role of snake venom proteases and tissue factor in venom induced hemostatic disorders and local hemorrhage in mice

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Role of snake venom proteases and tissue factor in venom induced hemostatic disorders and local hemorrhage in mice

Abstract

Bothrops jararaca venom (BjV) induces hemostatic and inflammatory manifestations. Tissue factor (TF) is essential for hemostasis, but aberrant TF expression can cause coagulopathy. We determined the effect of inhibition of BjV serine proteases and metalloproteinases, as well as host TF, on the systemic coagulopathy and local hemorrhage in envenomed mice. Venom serine proteases induced hypofibrinogenemia whereas venom metalloproteinases induced hemorrhage. TF did not contribute to venom-induced coagulopathy, but played a protective role in venom-induced bleeding in mice.
Sachetto, A. T., Mackman, N., & Santoro, M. L. (2026). Role of snake venom proteases and tissue factor in venom induced hemostatic disorders and local hemorrhage in mice. Toxicon, 109299. https://doi.org/10.1016/j.toxicon.2026.109299