Qualitative and Quantitative Proteomic Analysis of Venoms from Mexican Rattlesnakes

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  Qualitative and Quantitative Proteomic Analysis of Venoms from Mexican Rattlesnakes Abstract Despite the vast biodiversity of Mexican vipers, venom of endemic species has been barely studied. Here we analyzed the venom composition of three endemic species of rattlesnakes: Crotalus aquilus , C. triseriatus , and C. ravus . We used quantitative chromato-mass-spectrometry and compared venoms with C. molossus , a species commonly found in North America, in a comparative and phylogenetic framework. In total, we identified 165 proteins grouped in 19 main protein families, consistent with previous reports for viperid venoms. In C. aquilus and C. triseriatus , the most predominant protein-family type was Serine Proteases, and in C. triseriatus and C. molossus it was Snake Venom Metalloproteases. The Label-free quantification revealed a high proportion of Snake Venom Metalloproteases in C. aquilus , C. triseriatus , and C. molossus , reaching 28–47% of the total venom. In contrast, in ...

ERGA-BGE-CBP chromosome-level genome assembly of the blind scorpion Belisarius xambeui Simon, 1879 (Belisariidae, Scorpiones), the most singular scorpion in Europe

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ERGA-BGE-CBP chromosome-level genome assembly of the blind scorpion Belisarius xambeui Simon, 1879 (Belisariidae, Scorpiones), the most singular scorpion in Europe

Abstract

We present a chromosome-level reference genome for the blind scorpion Belisarius xambeui Simon, 1879 (Belisariidae, Scorpiones). The genome size estimated by flow cytometry (4.32 Gb) closely matches the final assembly size (3.98 Gb). The final assembly comprises 19,045 scaffolds, including 56 chromosome-level scaffolds (pseudochromosomes) that account for 90.08% of the total assembly. Nucleotide diversity across the genome was low, with an average π of 0.0018.

Guirao-Rico S, Pisarenco VA, Escuer P et al. ERGA-BGE-CBP chromosome-level genome assembly of the blind scorpion Belisarius xambeui Simon, 1879 (Belisariidae, Scorpiones), the most singular scorpion in Europe [version 1; peer review: awaiting peer review]. Open Res Europe 2026, 6:53 (https://doi.org/10.12688/openreseurope.22872.1)