New widow spider record of Latrodectus dahli Levi (Araneae: Theridiidae) extends known range in Northern Saudi Arabia

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  Image Credit: iNaturalist Photo 400937400, (c) Kian, some rights reserved (CC BY), uploaded by Kian, https://www.inaturalist.org/photos/400937400 New widow spider record of Latrodectus dahli Levi (Araneae: Theridiidae) extends known range in Northern Saudi Arabia ABSTRACT Researchers have documented the first confirmed occurrence of Dahl’s widow spider ( Latrodectus dahli vn Saudi Arabia’s Ha’il Region, extending the species’ known range into the country’s north. The finding fills an important gap in the spider’s Middle Eastern distribution and highlights how even medically important species remain under-documented in parts of the Arabian Peninsula. L. dahli Levi is a medically significant widow spider that is found in North Africa, Cyprus, Turkey, Azerbaijan, Kazakhstan, the Middle East, Iran and Central Asia. Prior to our work, there had been no records of it in northern Saudi Arabia although it had been found in central regions of Saudi Arabia such as Al Duwadimi, Riyadh and ...

A Hydrolase-Rich Venom Beyond Neurotoxins: Integrative Functional Proteomic and Immunoreactivity Analyses Reveal Novel Peptides in the Amazonian Scorpion Brotheas amazonicus

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A Hydrolase-Rich Venom Beyond Neurotoxins: Integrative Functional Proteomic and Immunoreactivity Analyses Reveal Novel Peptides in the Amazonian Scorpion Brotheas amazonicus

Abstract

The scorpion family Buthidae, renowned for its neurotoxin-rich venoms, dominates toxinology, while non-buthid venoms remain largely unexplored. Here, we present a comprehensive proteomic and biochemical characterization of the Amazonian chactid scorpion Brotheas amazonicus venom (BamazV), with emphasis on molecular complexity, proteolytic processing, and peptide diversity. Using an integrative venomics approach that combines molecular mass-based fractionation, reversed-phase chromatography, high-resolution mass spectrometry, N-terminal sequencing, and functional and immunological analyses, we reveal an unexpectedly complex venom profile enriched in high-molecular-weight components and extensively processed peptides, with more than 40 venom peptides sequenced by MS/MS and Edman degradation. The data provide evidence for non-canonical proteolytic events, including the generation of peptides from precursor regions not classically associated with mature venom components. In contrast to the venom of Tityus serrulatus, BamazV displays a “hydrolase-rich, neurotoxin-poor” profile, featuring a catalytically active Group III phospholipase A2 (BamazPLA2), a highly active hyaluronidase, metalloproteases, low-mass peptides, and potassium channel toxins. Our results suggest a hydrolytic prey-subjugation strategy, and limited cross-reactivity with commercial antivenom highlighted its distinct structural landscape. Overall, this study advances the understanding of venom evolution and proteolytic diversification in underexplored scorpion lineages, positioning B. amazonicus as a valuable model for investigating alternative venom strategies and identifying novel biotechnological scaffolds.

Wiezel, G. A., Bordon, K. D., Martins, J. G., Custódio, V. I., Matsuno, A. K., Procópio, R. E., & Arantes, E. C. (2026). A Hydrolase-Rich Venom Beyond Neurotoxins: Integrative Functional Proteomic and Immunoreactivity Analyses Reveal Novel Peptides in the Amazonian Scorpion Brotheas amazonicus. International Journal of Molecular Sciences, 27(3), 1475. https://doi.org/10.3390/ijms27031475