Venomics of the six-eyed sand spider, Sicarius rugosus (Araneae: Sicariidae), from the neotropical dry forest of Costa Rica
Venomics of the six-eyed sand spider, Sicarius rugosus (Araneae: Sicariidae), from the neotropical dry forest of Costa Rica
Venomics of the six-eyed sand spider, Sicarius rugosus (Araneae: Sicariidae), from the neotropical dry forest of Costa Rica Abstract Background Sicarius rugosus , the only member of the genus that inhabits Central America, is phylogenetically related to South American Sicarius spiders. These originated from a common ancestor with sister African species. Like Loxosceles , Sicarius exhibits venom phospholipase D activity due to a group of toxins known collectively as SicTox. Methods A gel-assisted, bottom-up, proteomic analysis was performed to characterize the venom composition of S. rugosus . Hyaluronidase activity was determined using zymography. Results We identified several SicTox sequences, all classified as β-clade paralogs and sharing unique peptides with proteins from S. patagonicus , S. peruensis, and other species. Enzymes such as metalloproteinases, including putative astacins, carboxypeptidases, and angiotensin-conv...
- Get link
- X
- Other Apps
