Venom Mass Fingerprints for Species Delimitation, Reloaded: A Test Case with Italian Scorpions (Euscorpius)

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  Venom Mass Fingerprints for Species Delimitation, Reloaded: A Test Case with Italian Scorpions (Euscorpius) Abstract Scorpion venoms are complex mixtures of bioactive compounds including proteins, peptides and enzymes. Although venoms exhibit substantial intraspecific variation, some compounds appear to be species-specific, highlighting their potential as chemotaxonomic markers. Venom mass fingerprints (MFPs) derived from Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry (MS) have been proposed as a means of delimiting species. However, their utility for species delimitation has rarely been evaluated against independent sources of taxonomic evidence. In the present study, the performance of MALDI-TOF MS-based venom peptide barcoding (mass ranges of m/z 800‒4500 and m/z 3000‒10,000) was assessed in 52 populations of eight Italian species of the scorpion genus Euscorpius Thorell, 1876, representing different levels of evolutionary divergenc...

Wildflower Strips Promote Spider Diversity and Biological Control Potential in a Semi-Arid Agroecosystem: Preliminary Insights from a Single Growing Season

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Wildflower Strips Promote Spider Diversity and Biological Control Potential in a Semi-Arid Agroecosystem: Preliminary Insights from a Single Growing Season

Abstract

Agricultural intensification degrades farmland biodiversity, prompting the use of wildflower strips to restore natural enemy populations. However, their spatio-temporal effects on predators in arid agroecosystems remain unclear. Therefore, we investigated ground-dwelling spiders in sown wildflower strips versus natural margins across four cropping systems in arid northwestern China using pitfall traps along a spatial gradient throughout the growing season. While the overall diversity did not differ significantly between treatments, three key patterns emerged: First, spider abundance remained stable across the spatial gradient, whereas taxonomic diversity increased significantly toward the crop interior. Second, while communities in natural margins peaked early and declined during the hot, dry mid-summer, wildflower strips maintained large populations, acting as crucial temporal refuges. Third, wildflower strips reorganized species co-occurrence networks, fostering more resilient community structures. We conclude that in water-limited environments, wildflower strips stabilize predator populations during harsh weather and enhance community resilience. These findings highlight the value of incorporating these strips when designing sustainable ecological pest management strategies for arid agroecosystems.

Tuo, M., Hu, W., Wu, D., Liu, W., Wang, S., Long, X., & Jin, Y. (2026). Wildflower Strips Promote Spider Diversity and Biological Control Potential in a Semi-Arid Agroecosystem: Preliminary Insights from a Single Growing Season. Insects, 17(7), 722. https://doi.org/10.3390/insects17070722