Venom composition of the Iberian adder (Vipera seoanei) from northern Portugal reveals limited sex and size variation

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  Venom composition of the Iberian adder (Vipera seoanei) from northern Portugal reveals limited sex and size variation Abstract Snake venom is a complex and dynamic ecological trait, with high potential for intraspecific variation. In this study, the venom composition of the Iberian adder ( Vipera seoanei ) from the Peneda Mountains of northern Portugal was investigated to explore whether venom varies with body size and sex. Venom proteomes from six individuals representing juvenile, subadult, and adult males and females were characterised using LC–MS/MS, while individual variation was assessed from SDS–PAGE profiles of 18 snakes. LC–MS/MS identified 144 proteins, dominated by phospholipases A 2 (PLA 2 ), snake venom serine proteases (svSP), and C-type lectin-related proteins (CTL). Descriptive comparisons revealed contrasting individual profiles, particularly between the juvenile male, characterised by high svSP and svMP abundance, and the juvenile female, characterised by high ...

Efficacy of neem oil (Azadirachta indica) as an antiparasitic treatment for scorpions (Androctonus bicolor) and its impact on venom production

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By Guy Haimovitch - Own work, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=630184

Efficacy of neem oil (Azadirachta indica) as an antiparasitic treatment for scorpions (Androctonus bicolor) and its impact on venom production

Abstract

Mite infestations are a recurrent welfare issue that significantly affect the health, venom production, and survival of scorpions, particularly under human care. Chemical acaricides such as permethrins and fipronil, widely used in veterinary medicine for mite and tick control, are contraindicated in scorpions. Since mites and scorpions both belong to the class Arachnida, even minimal exposure can result in severe toxicity or death. Neem oil (Azadirachta indica) has been widely recognized for its acaricidal, antibacterial, and antifungal properties. Its main compound, azadirachtin, interferes with mite development and reproduction, leading to reduced infestations and egg viability. This study evaluated the efficacy and safety of a 1% neem oil spray for controlling Pimeliaphilus spp. mites in forty adult Androctonus bicolor (A. bicolor) maintained for venom production. Neem oil was applied every 72 h for five sessions. Mite counts, egg viability, clinical tolerance, and venom productivity were assessed. Mite loads decreased progressively, reaching complete elimination by the fifth treatment, with no new mites detected during the subsequent 60 days. No mortality, irritation, or cuticular lesions were observed. Post-treatment venom extraction at six weeks, based on pooled venom, showed increases of 10% in liquid volume, 50% in dry mass, and 36.5% in protein concentration compared with pre-treatment values. These results demonstrate that 1% neem oil provides an effective, safe, sustainable and low-cost acaricidal option for scorpions under human care, with benefits for venom yield and animal welfare.
Planas, J., Moraleda-Berral, P., Checa, R., Mundanatt, V., Zahra, F., Salah, M., Bande, B., Sonavane, M., & Siyam, M. (2026). Efficacy of neem oil (Azadirachta indica) as an antiparasitic treatment for scorpions (Androctonus bicolor) and its impact on venom production. Veterinary Parasitology, 344, 110748. https://doi.org/10.1016/j.vetpar.2026.110748