First documented cases of invertebrate predation by greater grisons (Galictis vittata)

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  First documented cases of invertebrate predation by greater grisons (Galictis vittata) Abstract  We report the first documented predation of invertebrates by greater grisons (Galictis vittata) based on camera-trap footage from a mineral lick in Southeastern Peru. Two adult individuals were recorded capturing and consuming large arachnids, likely whip spiders (Amblypygi), from a natural cavity. One grison visibly removed and discarded part of the prey prior to ingestion, suggesting selective handling to reduce risk from defensive structures. These observations extend the known cavity-focused foraging behavior of G. vittata and indicate that amblypygids may represent an underreported but regular prey item in tropical habitats. Pottie, Sam, y Adrian Forsyth. 2026. «Primeros Registros Documentados De depredación De Invertebrados Por El grisón (Galictis Vittata)». Mammalogy Notes 12 (2), 608. https://doi.org/10.47603/mano.v12n2.608 .

Conserved Enzymatic Peptides in Bitis arietans Venom Revealed by Comparative Proteomics: Implications for Cross-Reactive Antibody Targeting

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Conserved Enzymatic Peptides in Bitis arietans Venom Revealed by Comparative Proteomics: Implications for Cross-Reactive Antibody Targeting

Abstract

Snakebite envenoming remains a critical public health issue, and the molecular variability of venoms limits the cross-species efficacy of conventional antivenoms. Here, we conducted a comparative proteomic analysis of Bitis arietans venom to identify conserved peptide regions derived from enzymatic toxins and evaluate their potential relevance for complementary immunotherapeutic applications. Enzyme-enriched venom fractions were isolated through sequential affinity and ion-exchange chromatography and were subsequently characterized using fluorogenic FRET substrates and inhibitor assays. LC–MS/MS analysis identified 1099 proteins and revealed 36 conserved peptides within snake venom metalloproteinases (SVMPs), serine proteases (SVSPs), and phospholipase A2 (PLA2), particularly located near catalytic residues and structurally essential motifs such as the HExxHxxGxxH zinc-binding site in SVMPs, the His-Asp-Ser catalytic triad in SVSPs, and the Ca2+-binding loop in PLA2, across Viperidae venoms. These conserved regions were also observed in homologous toxin isoforms from additional Viperidae genera, supporting the evolutionary conservation of key functional domains. While sequence conservation alone does not guarantee neutralization capacity, the identified regions represent strong candidates for structural epitope mapping and targeted antibody development. This study provides a peptide-level framework for advancing complementary antibody-based therapies designed to broaden cross-species toxin recognition, reduce antivenom dosage requirements, and improve clinical outcomes in snakebite envenoming.

Godoi, K. S., Portaro, F. C., Spencer, P. J., Vigerelli, H., & Silva, W. D. (2026). Conserved Enzymatic Peptides in Bitis arietans Venom Revealed by Comparative Proteomics: Implications for Cross-Reactive Antibody Targeting. International Journal of Molecular Sciences, 27(3), 1431. https://doi.org/10.3390/ijms27031431