Molecules, Morphometrics and Modeling of the Medically Important Genus Hemiscorpius Peters, 1861 (Scorpiones: Hemiscorpiidae) in Iran Reveal New Species from Kerman

 

Molecules, Morphometrics and Modeling of the Medically Important Genus Hemiscorpius Peters, 1861 (Scorpiones: Hemiscorpiidae) in Iran Reveal New Species from Kerman

Abstract

Eight species of the medically important scorpion genus Hemiscorpius Peters, 1861 have been reported in Iran, three of which are responsible for most of the severe clinical cases of envenomation. However, morphological similarity complicates species delimitation in this genus, hindering the identification of species implicated in envenomations. The present study integrates morphology, DNA sequences, and ecological niche modeling to clarify the taxonomy and distribution of Hemiscorpius in southern Iran, providing taxonomic insights relevant to public health and biodiversity conservation. Morphometric analyses were performed to evaluate size and shape differences; molecular phylogenetic analyses were conducted on DNA sequences of the mitochondrial Cytochrome c Oxidase Subunit I gene; and species distribution models, based on occurrence records and bioclimatic variables, were developed. Morphometric analyses revealed significant interspecific differences and sexual dimorphism. A new species was identified and described as Hemiscorpius aratta sp. n. Molecular phylogenetic analysis confirmed the distinctiveness of the new species and revealed intraspecific variation in the type species, Hemiscorpius lepturus Peters, 1861, suggesting possible cryptic diversity. Southern Iran, particularly the coastline of the Persian Gulf and the Gulf of Oman, represents a diversity hotspot for Hemiscorpius. Topographical barriers such as the Jebal Barez, Makkoran, and Zagros Mountain ranges promoted isolation and speciation, leading to high levels of endemism in the genus. Ecological niche models revealed that the distributions of Hemiscorpius species are strongly influenced by temperature and precipitation. Coastal species are restricted to thermally stable maritime habitats, whereas semi-arid species occupy regions with higher temperature seasonality. Range-restricted species are habitat specialists, vulnerable to environmental change. This study reinforces the importance of integrating morphological, molecular, and ecological data for resolving taxonomic ambiguity.