Clinical characteristics and long-term disabilities in children following Bothrops atrox envenomation in Manaus, western Brazilian Amazon

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  Image Credit: By Darío De la Fuente - https://www.inaturalist.org/photos/113840233, CC BY 4.0, https://commons.wikimedia.org/w/index.php?curid=129474300 Clinical characteristics and long-term disabilities in children following Bothrops atrox envenomation in Manaus, western Brazilian Amazon Abstract Background:   In the Brazilian Amazon, most snakebites are caused by Bothrops atrox . Although pediatric cases are less frequent, children are more vulnerable to severe complications and long-term disabilities. This study aims to describe the clinical profile of B. atrox envenomation in children treated at a tertiary hospital in Manaus, in Western Brazilian Amazon, and to characterize the resulting long-term musculoskeletal impairments in a subgroup of these patients. Methods:   We retrospectively analyzed sociodemographic and clinical data from patients up to 12 years and 11 months of age treated between January 2010 and December 2023. A total of 258 children who were ...

Targeted Metabolomics of Tityus Scorpion Venoms: Unveiling Small-Molecule Components

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Targeted Metabolomics of Tityus Scorpion Venoms: Unveiling Small-Molecule Components

Abstract

Scorpion venoms consist of proteins, peptides, and various low molecular weight (LMW) organic compounds, which act as toxins. Despite their potential significance, these compounds in scorpion venoms have been little investigated and their full range has not been well characterized. In this work, a targeted metabolomic approach was used in combination with an HPLC-QTOF-MS methodology to create a library of 55 LMW standard compounds, for the analysis of venoms from three Tityus species scorpions. This strategy enabled reliable identification of 45 compounds, including 20 amino acids, 4 organic acids, 12 biogenic amines, 6 nitrogenated bases and derivatives, 2 β-carboline-derived alkaloids, and 1 amphetamine. Most of the compounds identified were neurotransmitters and/or neurotoxins, while others can act as homeostasis disruptors or affect the diffusion of venom through the bodies of victims. Therefore, the LMW organic compounds in scorpion venoms play roles in the killing or paralyzing of prey, as well as in defense against large predators.

Targeted Metabolomics of Tityus Scorpion Venoms: Unveiling Small-Molecule Components
Nathalia Baptista Dias, Bibiana Monson de Souza, Geovanny Barroso, Javier Ortiz Leiva, Gabriela Mendonça Paula, Hipócrates M. Chalkidis, Valquíria Abrão Coronado Dorce, Osmar Malaspina, and Mario Sergio Palma, Journal of the American Society for Mass Spectrometry Article ASAP
DOI: 10.1021/jasms.5c00238