Spider venom peptides Ht1a and Gg1a are toxic to honeybee parasite Varroa destructor by topical application

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  Spider venom peptides Ht1a and Gg1a are toxic to honeybee parasite Varroa destructor by topical application Abstract Global food supply strongly depends on honeybee pollination services, which are threatened by insecticides and pests such as parasitic Varroa destructor mites. Chemical varroacides/acaricides are hampered by resistance development, necessitating the development of sustainable and environmentally friendly alternatives, with arthropod venom peptides being considered promising sources of acaricidal toxins. With only a few acaricidal venom peptides being reported, we performed a systematic topical screening of 50 arthropod venoms against V. destructor , with 78% of the venoms causing 100% mortality after 24 h. Deconvolution of the venoms from the Tasmanian cave spider Hickmania troglodytes and the Giant Japanese funnel-web spider Gigathele gigas led to identification of the varroacidal peptides Ht1a and Gg1a. Topical application of Ht1a and Gg1a reduced varroa mite ...

Proteomic profiling and biochemical characterization of enzymatic and non-enzymatic proteins of Heterometrus bengalensis venom and their pathophysiological functions in the Swiss albino mice model

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Proteomic profiling and biochemical characterization of enzymatic and non-enzymatic proteins of Heterometrus bengalensis venom and their pathophysiological functions in the Swiss albino mice model

Abstract

Due to its significant mortality and morbidity, scorpion sting is a public health concern worldwide, particularly in tropical and subtropical regions. Heterometrus bengalensis (HB) significantly impacts health; however, HB's venom composition is the least characterized. The present study provides a comprehensive insight into the HB venom (HBV) profile by in vitro enzyme assay and tandem mass spectrometry analysis of SDS-PAGE-separated venom proteins and peptides. HBV showed calcium-dependent PLA2 and nucleotidase (ATPase, ADPase, and AMPase) activities, indicating myotoxicity and ATP-depletion-induced shock. Proteomic analysis of HBV identified 25 toxins belonging to 8 protein toxin families from searching the MS data against the Scorpionidae family (taxid 6888) of toxin entries, which collectively contribute to the pharmacological effects of HBV. The lethal dose of HBV in Swiss albino mice (SAM) was determined to be 20 mg/kg (i.v). In vivo, HBV-envenomed SAM revealed severe physiological disturbances, elevated liver enzymes, plasma glucose levels, histopathological evidence of organ damage, and myotoxicity. The serum proinflammatory cytokines (IL-1β, IL-6, and TNFα) in HBV-injected SAM were significantly increased (1–2 fold) compared to the controls. The current findings underscore the complexity of HBV, highlighting the urgent need for improved therapeutic strategies, including the development of species-specific and targeted antivenom.
Nath, S., Mahato, R., Kakati, H., & Mukherjee, A. K. (2025). Proteomic profiling and biochemical characterization of enzymatic and non-enzymatic proteins of Heterometrus bengalensis venom and their pathophysiological functions in the Swiss albino mice model. International Journal of Biological Macromolecules, 146181. https://doi.org/10.1016/j.ijbiomac.2025.146181