Novel Biochemical Findings from Bothrops marajoensis Venom Abstract Bothrops marajoensis is a Brazilian pit viper whose venom remains poorly characterized compared with those of other medically important Bothrops species. In the present study, we performed a comparative biochemical characterization of B. marajoensis venom with six additional Bothrops venoms ( B. alternatus , B. atrox , B. jararaca , B. jararacussu , B. moojeni , and B. neuwiedi ). Venoms were analyzed by reversed-phase chromatography, electrophoresis, immunorecognition by commercial bothropic antivenom, phosphodiesterase activity, and their effects on the viability of differentiated C2C12 myotubes. Chromatographic and electrophoretic analyses revealed a largely conserved overall venom composition among species, with qualitative differences, although quantitative differences in protein relative abundance were evident. Bothropic antivenom recognized all venoms, indicating broad antigenic cross-reactivity despit...
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