Yinanyphaena gen. nov., a new ghost spider genus with descriptions of five new species and seven new combinations from China (Araneae, Anyphaenidae)

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  Yinanyphaena gen. nov., a new ghost spider genus with descriptions of five new species and seven new combinations from China (Araneae, Anyphaenidae) Abstract A new genus, Yinanyphaena Wang & Mi, gen. nov ., of the family Anyphaenidae Bertkau, 1878, is proposed based on molecular phylogenetic results and morphological evidence. Five new species collected from the Qomolangma National Nature Reserve, Xizang, China are described: Y. haochuni Wang & Mi, sp. nov . (♂♀), Y. jinhuai Wang & Mi, sp. nov . (♂♀), Y. qomolangma Wang & Mi, sp. nov . (♂♀), Y. shikaii Wang & Mi, sp. nov . (♀), and Y. xufeii Wang & Mi, sp. nov . (♀). In addition, seven new combinations transferred from Anyphaena Sundevall, 1833 are also proposed: Y. cibagou (Wang & Mi, 2024), comb. nov ., Y. linzhi (Wang & Mi, 2024), comb. nov ., Y. rhynchophysa (Feng, Ma & Yang, 2012), comb. nov ., Y. shufui (Wang & Mi, 2024), comb. nov ., Y. tibet (Lin & Li, 202...

GLP-1 receptor agonist properties of a chimeric peptide derived by hybridization of Latrodectus αLatrotoxin and Heloderma Exendin-4

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GLP-1 receptor agonist properties of a chimeric peptide derived by hybridization of Latrodectus αLatrotoxin and Heloderma Exendin-4

Abstract

Chimeric peptides comprised of amino acid sequence motifs found within hormones, neuropeptides, and insect or lizard toxins are now under investigation for their potential use in therapeutics. Here, we report the discovery of one such peptide designated as Black Widow Spider-Exendin-4 (BW-Ex-4). It consists of a putative G protein-coupled receptor (GPCR) binding domain present within αLatrotoxin (αLTX) isolated from Latrodectus, and fused to N- and C- terminal motifs found within the glucagon-like peptide-1 receptor (GLP-1R) agonist Exendin-4 isolated from Heloderma. FRET reporter assays that monitor cAMP production establish BW-Ex-4 to be a specific GLP-1R agonist without any stimulatory action at glucose-dependent insulinotropic peptide (GIP), glucagon, or corticotropin releasing hormone (CRH) receptors. Structural modeling studies of the predicted BW-Ex-4 binding sites at GPCRs of Family B provide new insights concerning the molecular basis for chimeric peptide stimulatory actions at the GLP-1R. We also report that BW-Ex-4 acts in obese hyperglycemic Leprdb/db mice to suppress appetite, lower body weight, improve glucoregulation, and to reduce circulating levels of pro-inflammatory cytokines. Collectively, these findings establish combinatorial chimeric peptide chemistry in which αLTX serves as a molecular scaffold for the design of hybrid peptides with novel GPCR stimulating properties.
Chepurny, O. G., Liles, A. N., Cham, N., Matsoukas, M., Liapakis, G., Meng, Q., Cooney, R. N., Doyle, R. P., & Holz, G. G. (2025). GLP-1 receptor agonist properties of a chimeric peptide derived by hybridization of Latrodectus αLatrotoxin and Heloderma Exendin-4. General and Comparative Endocrinology, 114745. https://doi.org/10.1016/j.ygcen.2025.114745