Six Months of Prompt Engineering: Building Scientific Altitudinal, Topographical, and Geological Visualizations for Spiders

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  Six Months of Prompt Engineering: Building Scientific Altitudinal, Topographical, and Geological Visualizations for Spiders By: Luis A. Roque,  Arácnido Taxonomy Six months ago, I set out on what seemed like a relatively straightforward goal: create better visual representations of where spiders live. What I quickly discovered was that producing scientifically meaningful ecological visualizations requires far more than simply asking artificial intelligence to draw a landscape. It requires learning how to communicate ecology, geology, geography, climate, and biodiversity in a language that AI can understand. Over the past six months, I have spent hundreds of hours developing, testing, refining, and rewriting prompts designed to generate publication-quality altitudinal, topographical, geological, and habitat-based visualizations for spiders, particularly tarantulas and other species whose distributions are closely tied to specific environmental conditions. What began as a curi...

Evaluation of Anticancer and Antimicrobial Potentials of Tarantula cubensis Venom (Theranekron® D6)

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Evaluation of Anticancer and Antimicrobial Potentials of Tarantula cubensis Venom (Theranekron® D6)

Abstract
Tarantula cubensis venom (Theranekron®D6) is widely used in veterinary medicine as a drug with anti-tumor, wound healing, anti-inflammatory, antiphlogistic properties. The purpose of the study was to explore Theranekron®D6 (TD6)'s antibacterial activity and its impact on apoptotic cell death in human colon and liver cancer cell lines. TD6 showed a dose and time dependent cytotoxic effect at 12h and 24h in HT-29 and HUH7 cancer cell lines. The IC50 values of TD6 were calculated as 12.18 µg/mL and 25.10 µg/mL in HT-29 and HUH-7 cell lines at 24h, respectively. TD6 induced apoptosis in HT-29 and HUH-7 cell lines. In these cells exposed to TD6, while the BAX/BCL-2 ratio and CASP-3 mRNA level increased, the HSP90 mRNA and protein level decreased. Also, TD6 exhibited antimicrobial activities against Pseudomonas aeruginosa, Enterococcus faecalis, Klebsiella pneumonia, Staphylococcus aureus, Candida albicans and Candida utilis. Obtained results demonstrated that TD6 has great potentials as alternative therapeutic for cancer and infectious diseases as apoptosis inducer and antimicrobial agent. 

Gökşen Tosun, N., Kaplan, Ö., & Özgür, A. (2024). Evaluation of Anticancer and Antimicrobial Potentials of Tarantula cubensis Venom (Theranekron® D6). Erzincan University Journal of Science and Technology, 17(3), 626-637. https://doi.org/10.18185/erzifbed.1463282