Herpetological misinformation and sensationalism: media framing on biological and ecological aspects of the golden lancehead (Bothrops insularis)

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  Herpetological misinformation and sensationalism: media framing on biological and ecological aspects of the golden lancehead (Bothrops insularis) Abstract The internet is currently one of the most important means of communication, which may include the circulation of misinformation. Snakes have always been a target of the sensationalist media, being pictured as scary and aggressive animals to be killed indiscriminately by humans. Such fear based on misinformation may compromise species conservation. Here, we address the spread of misinformation about Bothrops insularis and Queimada Grande Island in digital media, highlighting the negative impact on public perception and species conservation. The analysis of 230 online sources revealed that approximately 94% of them contained false information, many using sensationalist language, especially regarding the toxicity of the venom and the population density of the snake, as well as bringing contents that did not align with scientific d...

Similar neurotoxin expression profiles of traditional Chinese scorpion medicine material between juvenile and adult Mesobuthus martensii scorpions revealed by multiple strategic proteomics

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Similar neurotoxin expression profiles of traditional Chinese scorpion medicine material between juvenile and adult Mesobuthus martensii scorpions revealed by multiple strategic proteomics

Abstract

Ethnopharmacological relevance

The Mesobuthus martensii scorpions, called as “Quanxie”, are known Chinese medicinal material base on the “Combat poison with poison” strategy for more than one thousand years, and still widely used to treat various diseases according to the the Pharmacopoeia of the People’s Republic of China nowadays.

Aim of study

The study aims to investigate the similarity of scorpion neurotoxins at the protein level between the juvenile and adult Mesobuthus martensii scorpions as Chinese medicine materials.

Materials and methods

The second-, third- and fourth-instar, and adult Mesobuthus martensii scorpions were collected for the characterization of neurotoxin expression through multiple strategic proteomics, including undigested scorpion venom, endopeptidase-digested, and undigested scorpion telson extract for the sample analysis.

Results

Based on the known 107 scorpion neurotoxins from the genomic and transcriptomic analysis of adult Mesobuthus martensii scorpions, the multiple strategic proteomics first revealed that neurotoxins exhibited more stability in telson extract than secreted venom. In the reported transcripts of scorpion neurotoxins, approximately 53%, 56%, 66% and 78% of neurotoxins were detected through undigested scorpion venom, the endopeptidase Arg-C-, Lys-C-digested telson extract, and undigested telson extract strategies, respectively. Nearly 79% of scorpion neurotoxins detected in third-instar Mesobuthus martensii scorpions represent the largest number of scorpion neurotoxins from proteomic analysis to date. Moreover, a total of 84% of scorpion neurotoxins were successfully identified at the protein level, and similar neurotoxin expression profiles in second-, third- and fourth-instar, and adult Mesobuthus martensii scorpions were first revealed by the multiple strategic proteomics.


Guo, Y., Zhu, W., Yuan, P., Huang, X., Lu, S., Cao, Z., Zhao, X., & Wu, Y. (2024). Similar neurotoxin expression profiles of traditional Chinese scorpion medicine material between juvenile and adult Mesobuthus martensii scorpions revealed by multiple strategic proteomics. Journal of Ethnopharmacology, 118338. https://doi.org/10.1016/j.jep.2024.118338