The distribution, spatial clustering, and density of trapdoor spiders in two California coastal dune ecosystems

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  The distribution, spatial clustering, and density of trapdoor spiders in two California coastal dune ecosystems Abstract Coastal dune ecosystems support endemic trapdoor spiders, yet population data for these species are limited. We conducted the first coastal dune trapdoor spider population estimation surveys at Moss Landing State Beach (California) and for Aptostichus stephencolberti Bond 2008 at Sunset State Beach using randomly selected 1m 2 quadrats across ~26,000 m 2 per site. Burrow densities were similar between sites (Sunset: 0.287 burrows/m 2 ; Moss: 0.453 burrows/m 2 ), yielding estimated population sizes of ~35,206 and ~30,959 individuals, respectively. However, spatial analyses revealed contrasting patterns of burrow distribution. At Sunset State Beach, burrow presence was strongly associated with vegetation and occurred closer to plants, despite vegetation being randomly distributed. In contrast, no vegetation–burrow association was detected at Moss Landing, alth...

Evaluation of the immunoprotective power of a multiple antigenic peptide against Aah II toxin of Androctonus australis hector scorpion

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Evaluation of the immunoprotective power of a multiple antigenic peptide against Aah II toxin of Androctonus australis hector scorpion

Abstract

Scorpion envenoming (SE) is a public health problem in developing countries. In Algeria, the population exposed to the risk of SE was estimated at 86.45% in 2019. Thus, the development of a vaccine to protect the exposed population against scorpion toxins would be a major advance in the fight against this disease.

This work aimed to evaluate the immunoprotective effect of a Multiple Antigenic Peptide against the Aah II toxin of Androctonus australis hector scorpion, the most dangerous scorpion species in Algeria. The immunogen MAP1Aah2 was designed and tested accordingly. This molecule contains a B epitope, derived from Aah II toxin, linked by a spacer to a universal T epitope, derived from the tetanus toxin.

The results showed that MAP1Aah2 was non-toxic despite the fact that its sequence was derived from Aah II toxin. The immunoenzymatic assay revealed that the 3 immunization regimens tested generated specific anti-MAP1Aah2 antibodies and cross-reacted with the toxin. Mice immunized with this immunogen were partially protected against mortality caused by challenge doses of 2 and 3 LD50 of the toxin. The survival rate and developed symptoms varied depending on the adjuvant and the challenge dose used. In the in vitro neutralization test, the immune sera of mice having received the immunogen with incomplete Freund’s adjuvant neutralized a challenge dose of 2 LD50.

Hence, the concept of using peptide dendrimers, based on linear epitopes of scorpion toxins, as immunogens against the parent toxin was established. However, the protective properties of the tested immunogen require further optimizations.


Benazzouz, S. M., Benlouahmia, N., Bouhadida, K., Benlamara, M., Arezki, N., El Kheir Sadeddine, O., Issad, M., Attal, N., Mansouri, K., Derrar, F., & Djidjik, R. (2024). Evaluation of the immunoprotective power of a multiple antigenic peptide against Aah II toxin of Androctonus australis hector scorpion. Vaccine: X, 100503. https://doi.org/10.1016/j.jvacx.2024.100503