Hydrophobic Interaction Chromatography Coupled to High-Throughput Venomics for Snake Venom Characterisation with Parallel Bioactivity Assessment
Hydrophobic Interaction Chromatography Coupled to High-Throughput Venomics for Snake Venom Characterisation with Parallel Bioactivity Assessment
Hydrophobic Interaction Chromatography Coupled to High-Throughput Venomics for Snake Venom Characterisation with Parallel Bioactivity Assessment Abstract Between 80,000 and 140,000 fatalities occur yearly because of snakebite envenoming, which causes it to be classified as a Neglected Tropical Disease (NTD), stressing the importance of snake venom toxin characterisation to aid the development of next-generation antivenoms. Snake venom is made up of a mixture of bioactive proteins that are also known as venom toxins. These venom toxins, being complex and structurally diverse, present a significant challenge for analytical separation and functional characterisation. Traditional methods, such as reversed-phase liquid chromatography (RPLC), often involve denaturing conditions that can alter the native state of these proteins, potentially reducing their bioactivity and limiting subsequent bioassays. Therefore, this study developed and optimised an analytical methodology using non-dena...
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