Qualitative and Quantitative Proteomic Analysis of Venoms from Mexican Rattlesnakes

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  Qualitative and Quantitative Proteomic Analysis of Venoms from Mexican Rattlesnakes Abstract Despite the vast biodiversity of Mexican vipers, venom of endemic species has been barely studied. Here we analyzed the venom composition of three endemic species of rattlesnakes: Crotalus aquilus , C. triseriatus , and C. ravus . We used quantitative chromato-mass-spectrometry and compared venoms with C. molossus , a species commonly found in North America, in a comparative and phylogenetic framework. In total, we identified 165 proteins grouped in 19 main protein families, consistent with previous reports for viperid venoms. In C. aquilus and C. triseriatus , the most predominant protein-family type was Serine Proteases, and in C. triseriatus and C. molossus it was Snake Venom Metalloproteases. The Label-free quantification revealed a high proportion of Snake Venom Metalloproteases in C. aquilus , C. triseriatus , and C. molossus , reaching 28–47% of the total venom. In contrast, in ...

Integrative systematics of the widespread Middle Eastern buthid scorpion, Hottentotta saulcyi (Simon, 1880), reveals a new species in Iran

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Integrative systematics of the widespread Middle Eastern buthid scorpion, Hottentotta saulcyi (Simon, 1880), reveals a new species in Iran

Abstract

Morphological and genetic variation among populations of the widespread buthid scorpion, Hottentotta saulcyi (Simon, 1880), occurring in western and southwestern Iran was explored using morphometric variables, one nuclear marker (28S rDNA) and three mitochondrial markers (12S rDNA, 16S rDNA, and Cytochrome c Oxidase Subunit I). Genetic and morphometric statistical analyses revealed extensive cryptic diversity. Phylogenetic analysis with Bayesian Inference and Maximum Likelihood uncovered two divergent clades, one of which is described as a new species, Hottentotta hatamtiorum sp. nov., from Ilam and Khuzestan Provinces, southwestern Iran. The description of the new species raises the total count of Hottentotta Birula, 1908 species to 61, twelve of which are endemic or subendemic to the Iranian Plateau.

Amiri M, Prendini L, Hussen FS, Aliabadian M, Siahsarvie R, Mirshamsi O (2024) Integrative systematics of the widespread Middle Eastern buthid scorpion, Hottentotta saulcyi (Simon, 1880), reveals a new species in Iran. Arthropod Systematics & Phylogeny 82: 323-341. https://doi.org/10.3897/asp.82.e98662