Snakebite knowledge among healthcare workers in Gabon: A health facility-based cross-sectional survey

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  Snakebite knowledge among healthcare workers in Gabon: A health facility-based cross-sectional survey Abstract Background Snakebite envenoming remains a neglected health issue in many countries, including Gabon, where the limited availability of snakebite-specific training, clinical guidelines, and essential resources at health facilities may lead to gaps in healthcare workers’ knowledge and confidence and the use of non-recommended treatment practices. This study aimed to assess healthcare workers’ knowledge of snakebite management in the Ogooué et des Lacs department of Moyen-Ogooué province in Gabon. Methods From June to August 2023, we conducted a cross-sectional survey targeting all healthcare workers in Ogooué et des Lacs who may be involved in snakebite management. We collected information on prior training, self-perceived knowledge of snakebite management, symptom recognition, clinical management practices, and snake identification. Knowledge was assessed using 10 true/fa...

Yellow scorpion (Buthus sinidicus) venom peptides induce mitochondrial-mediated apoptosis in cervical, prostate and brain tumor cell lines

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Yellow scorpion (Buthus sinidicus) venom peptides induce mitochondrial-mediated apoptosis in cervical, prostate and brain tumor cell lines


Abstract

Scorpion venoms are known to contain over 100,000 biologically active constituents. However, only a few of them have been studied. The major constituents of venom are proteins and peptides, which exhibit various biological and pharmacological properties, including anticancer activities. In the current study, the venom of yellow scorpions (Buthus sindicus) found in Sindh, Pakistan, was extracted and evaluated for its anti-cancer and anti-inflammatory activities. The crude venom showed a dose dependent inhibition of phagocyte oxidative burst from human whole blood cells (28.3% inhibition at highest tested concentration of 300 μg/mL). In-vitro cytotoxicity of crude venom was evaluated against human prostrate (PC3), cervical (HeLa) and neuroblastoma (U87-MG) cell lines, along with cytotoxicity against normal human fibroblast (BJ) cells. Crude venom was cytotoxic to all cell lines, with prominent inhibitory effect on PC3 cells. Crude venom was fractionated through RP-UPLC, resulted in fifteen fractions, followed by evaluation of their anticancer potential. Among all, the fraction I significantly (P < 0.001) reduced the cell viability of all three cancer cell lines, and exhibited insignificant cytotoxicity against normal cell line. Furthermore, the apoptotic cell death pathway was evaluated for crude venom, and fraction I, in most sensitive cell line PC3, by using flow-cytometry analysis. Both crude venom and its fraction I caused a mitochondrial-mediated apoptosis in prostate cancer cells (PC3). To the best of our knowledge, this is the first report of the anticancer and anti-inflammatory activity of venom of Pakistani yellow scorpions. Results indicate their therapeutic potential, and a case for further purification and validation studies.

Hassan, H., Mirza, M. R., Jabeen, A., Alam, M., Kori, J. A., Sultan, R., & Choudhary, M. I. (2024). Yellow scorpion (Buthus sinidicus) venom peptides induce mitochondrial-mediated apoptosis in cervical, prostate and brain tumor cell lines. PLOS ONE, 19(2), e0296636. https://doi.org/10.1371/journal.pone.0296636