A reassessment of “Tityus” tenuimanus Banks, 1910, as a misidentified Leiurus sp. (Scorpiones: Buthidae)

Posted by
  A reassessment of “Tityus” tenuimanus Banks, 1910, as a misidentified Leiurus sp. (Scorpiones: Buthidae) Abstract This study re-examines the holotype of “Tityus” tenuimanus Banks, 1910, previously synonymized with Buthus atlantis Pocock, 1889 by Lourenço & Francke (1984). Our results indicate that the specimen is a misidentified representative of Leiurus Ehrenberg, 1828, as it exhibits the diagnostic characters of this genus: the central lateral and posterior median carinae of the carapace fused into a lyre-shaped configuration, and tergites I–II bearing five granular carinae (a character absent in the genus Buthus Leach, 1815). Moreover, the holotype lacks one of the most distinctive characters of the genus Tityus Koch, 1836, namely the presence of a subaculear tubercle. The holotype shares several morphological features with two species described from Egypt (especially trichobothrial configuration on the fixed finger of pedipalp), Leiurus libycus (Birula, 1908) a...

Integration of proteomic and transcriptomic data of the venom and venom gland from Tityus jaimei

Posted by

 


Integration of proteomic and transcriptomic data of the venom and venom gland from Tityus jaimei

Abstract

This work presents a comprehensive transcriptomic and proteomic analysis of the venom gland and venom of Tityus jaimei, a recently described scorpion species of medical relevance in Panama and Costa Rica. High-throughput RNA sequencing (RNA-seq) and tandem mass spectrometry (MS/MS) enabled the identification of a diverse repertoire of venom proteins. Notably, this is the first report of proteins belonging to the nucleotide pyrophosphatase/phosphodiesterase and knottins families in the venom proteome of a scorpion from the genus Tityus. In addition, several known venom protein families were identified, including hyaluronidases, voltage-gated sodium and potassium channel toxins, lipolysis-activating peptides (LVPs), cysteine-rich secretory proteins (CRISPs), metalloproteinases, peptidylglycine alpha-hydroxylating monooxygenases (PHMs), serine proteases, alpha-amylases, single insulin-like growth factor-binding domain proteins, non-disulfide-bridged peptides (NDBPs), chitinases, cyclotide trypsin inhibitors, and calcin-like peptides. The identification of 16 distinct families in the venom of Tityus jaimei offers novel insights into its composition and the diversity of Tityus venoms in Central America. Finally, the use of IA to protein domain search for protein annotation.
Escobar, A., Salazar, M. H., Hernández-Ortiz, M., Clement, H., Encarnación, S., Cleghorn, J., Acosta, H., & Corzo, G. (2025). Integration of proteomic and transcriptomic data of the venom and venom gland from Tityus jaimei. Journal of Proteomics, 105543. https://doi.org/10.1016/j.jprot.2025.105543