Qualitative and Quantitative Proteomic Analysis of Venoms from Mexican Rattlesnakes

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  Qualitative and Quantitative Proteomic Analysis of Venoms from Mexican Rattlesnakes Abstract Despite the vast biodiversity of Mexican vipers, venom of endemic species has been barely studied. Here we analyzed the venom composition of three endemic species of rattlesnakes: Crotalus aquilus , C. triseriatus , and C. ravus . We used quantitative chromato-mass-spectrometry and compared venoms with C. molossus , a species commonly found in North America, in a comparative and phylogenetic framework. In total, we identified 165 proteins grouped in 19 main protein families, consistent with previous reports for viperid venoms. In C. aquilus and C. triseriatus , the most predominant protein-family type was Serine Proteases, and in C. triseriatus and C. molossus it was Snake Venom Metalloproteases. The Label-free quantification revealed a high proportion of Snake Venom Metalloproteases in C. aquilus , C. triseriatus , and C. molossus , reaching 28–47% of the total venom. In contrast, in ...

Fatal Bothrops atrox snakebite with thrombotic microangiopathy, hemorrhagic stroke and acute kidney injury

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By Darío De la Fuente - https://www.inaturalist.org/photos/113840233, CC BY 4.0, https://commons.wikimedia.org/w/index.php?curid=129474300

Fatal Bothrops atrox snakebite with thrombotic microangiopathy, hemorrhagic stroke and acute kidney injury

Abstract

Bothrops atrox is responsible for most snakebite envenomations in the Amazon region. Its venom induces systemic hemostatic disturbances and local tissue damage, potentially leading to severe complications. This report describes a fatal case of B. atrox envenomation in a 53-year-old male with a history of hypertension and alcoholism. The patient presented with persistent incoagulable blood, thrombocytopenia, anemia, and progressive renal dysfunction. These findings were consistent with venom-induced consumption coagulopathy (VICC) associated with thrombotic microangiopathy (TMA). He subsequently developed intracranial hemorrhage, the autopsy revealed a confirmed subarachnoid hemorrhage, and acute kidney injury (AKI), which contributed to systemic decompensation. Postmortem findings included subarachnoid hemorrhage, acute tubular necrosis, hepatic steatosis, and fibrin thrombi in cutaneous vessels. This case highlights the potential for severe systemic involvement in Bothrops atrox envenomation, particularly in patients with preexisting comorbidities, and underscores the need for early recognition of complications such as VICC and TMA to guide appropriate clinical management.
Duarte, L. R. A., Carvalho, É., Melo, T. F. C., Barbosa, F. B. A., De Andrade, R. V., Sartim, M., Araújo, F. Q., Sachett, J., Pivoto, G., De Lima Ferreira, L. C., & Monteiro, W. (2025). Fatal Bothrops atrox snakebite with thrombotic microangiopathy, hemorrhagic stroke and acute kidney injury. Toxicon, 268, 108610. https://doi.org/10.1016/j.toxicon.2025.108610